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Muse® Autophagy LC3-Antibody Based Kit

Muse® Autophagy LC3-Antibody Based Kit

MCH200109


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Description

This kit contains two key detection reagents to facilitate the monitoring of lipidated LC3-II in a given cell system, and works by:1. Employing a selective permeabilization solution to discriminate cytosolic LC3 from autophagic LC3 by extracting the soluble cytosolic proteins while protecting LC3, which has been sequestered into the autophagosome. 2. Utilizing an autophagy detection reagent (Autophagy Reagent A) to prevent the lysosomal degradation of LC3, allowing for its quantification by flow cytometry

Additional details
  • selective
  • pathway
  • protocol
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