Muse® Bcl-2 Activation Dual Detection Assay Kit

The Muse Bcl-2 Activation Dual Detection Kit includes two directly conjugated antibodies—a phospho-specific anti-phospho-Bcl-2 (Ser70)-Alexa Fluor® 555 and an anti-Bcl-2-PECy5 conjugated antibody—to measure total levels of Bcl-2 expression. This two-color kit is designed to detect Bcl-2 pathway activation by measuring Bcl-2 phosphorylation relative to the total Bcl-2 expression in any given cell population. By doing this, the levels of both the total and phosphorylated protein can be measured simultaneously in the same cell, resulting in a normalized and accurate measurement of Bcl-2 activation after treatment. Moreover, simultaneous measurement of both total and phospho-Bcl-2 confirms target specificity of the phosphorylation event. Together, a total and phospho antibody duo performed in multiplex provides enhanced and more reliable detection of the phospho:total ratio within a mixed cell population. Muse System provides statistical values, measuring the Percentage of inactivated cells / activated cells (via phosphorylation) / non-expressing cells