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Muse® H2A.X Activation Dual Detection Kit

Muse® H2A.X Activation Dual Detection Kit

MCH200101


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Description

Includes two directly conjugated antibodies—a phospho-specific anti-phospho-Histone H2A.X (Ser139)-Alexa Fluor® 555 and an anti-Histone H2A.X-PECy5 conjugated antibody—to measure total levels of Histone H2A.X. This two-color kit is designed to detect Histone H2A.X pathway activation by measuring H2A.X phosphorylation relative to the total H2A.X expression in any given cell population. By doing this, the levels of both the total and phosphorylated protein can be measured simultaneously in the same cell, resulting in a normalized and accurate measurement of H2A.X activation after stimulation.Get Percentage of inactivated cells/activated cells (via phosphorylation)/ non-expressing cells。

Additional details
  • pathway
  • protocol
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